TABLE 1.
Yeast strains and plasmids used in this study
| Yeast strain | Host genotype | Plasmid(s) | Description of plasmid(s) | Reference |
|---|---|---|---|---|
| AG51 | YH51 (MATaGAL+ ura3-52 spt3-202 his4-539 lys2-801) | pJEF724 (pGTy1-H3) | 2μm URA3-marked plasmid containing galactose-inducible wild-type Ty1-H3 | 21 |
| AG53 | YH51 | pGTyΔSal (pGTy1-H3ΔTYB) | Same as pJEF724 but with most of POL open reading frame deleted | 21 |
| AG1583 | YH51 | pJEF1076 | Same as pJEF724 but containing Ty1-173 from XhoI to BstEII | 4 |
| AG1585 | YH51 | pJEF1510 | Same as pJEF724 but Ty2-H556 | 3a |
| AG331 | MATα ura3-52 trp1Δ1 leu2-3,112 imt1::TRP1 imt2::TRP1 imt3::TRP1 imt4::TRP1 | pKC35 + pKC66 | Same as pJEF724 but containing PBS mutations and a neor marker + cen plasmid containing wild-type IMT4 | 10 |
| YH10 | MATa GAL+ ura3-52 his4-539 lys2-801 | None | 54 |