Figure 4. Effects of dLAN on contusion SCI-mediated neuropathic pain symptoms.

A. SCI induced hypersensitivity to heat from 7–28 dpo as assessed by the Hargreaves test. There was no significant difference in heat sensitivity between LD or dLAN groups. B. Plot of individual withdrawal latencies to heat at 7 dpo to illustrate post-surgery difference of SCI vs Sham animals. C. SCI elicited hypersensitivity to mechanical stimulation as assessed by the von Frey test. SCI-dLAN mice showed a transient increase in mechanical sensitivity at 13 dpo compared to SCI-LD mice. D. Plot of individual SUDO filament thresholds at 13 dpo. Sham-LD: n=6; Sham-dLAN: n=7; SCI-LD: n=10; SCI-dLAN: n=8. Plots show mean ± SEM. Significant (p < 0.05) surgery (red cross) x dpo (hourglass) interactions were observed via a three-way ANOVA. Timepoints with significantly different SCI vs. Sham results—as determined by Holm-Sidak post-hoc testing—are indicated with a red cross, while significant SCI-LD vs SCI-dLAN differences are indicated by an asterisk.