TABLE 1.
RT activities of different combinations of genetically engineered HTLV-1 Pol proteins
| Content of protein extract no.:
|
Amt of [3H]dGMP incorporated (pmol)a | |
|---|---|---|
| 1 | 2 | |
| RT | —b | <0.1 |
| RT-RH | — | 0.6 |
| RT-RH-IN | — | 0.5 |
| RT | RT-RH | <0.1 |
| RT | RT-RH-IN | 0.1 |
| RT-RH | RT-RH-IN | 14.8 |
| MuLV RTc | — | 55.4 |
The enzyme assays were carried out as described in Materials and Methods, using 20 μl of extract no. 1 plus 20 μl of extract no. 2.
—, 20 μl of mock extract.
MuLV RT (40 U) was tested under the same conditions.