Fig. 4.

Increased CD8 T-cell influx and activity in TME with RT + IL7 treatment. A Experimental schematic showing timepoints of treatment. Mice were treated with two fractions of 4 Gy RT and two weeks of i.p. IL7. Flow cytometry was performed on day 22 after completion of IL7 course. B–F Results of flow cytometry (n = 4 for RT, n = 5 for all other cohorts). Flow cytometry results were gated for infiltrating lymphocytes, proliferation markers, and effector function. G Differences in CD127 expression between Tregs, conventional CD4 T-cells, and CD8 T-cells. H Immunofluorescence staining verifying colocalization of CD8 and CD127. Yellow arrow showing a representative example of co-staining. I Bulk tumor RNA sequencing showing upregulation of chemokine release in RT treated mice (n = 3) three days post RT compared to untreated mice (n = 3). Statistics were computed using one-way ANOVA (B–G). The mean ± SEM is shown (B–G). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001