Fig. 6.

IL7 in combination with RT stimulates precursor T-cell development in the bone marrow. A Experimental schematic showing timing of treatments. Two doses of 4 Gy RT and two weeks of IL7 via i.p. injections were given. Bone marrow was harvested on day 22 following completion of treatment. B–D Results of bone marrow flow cytometry (n = 5 for each cohort) with lineage tree tracing. E Gating strategy used for identification of hematopoietic stem cell (HSC, Lin⁻Sca1⁺cKit⁺CD34⁻CD150⁺), common lymphoid progenitor (CLP, Lin⁻Sca1⁺cKit⁺CD127⁺), and early thymic progenitor (ETP, Lin⁻Sca1⁺cKit⁺CD127⁺B220⁻). Statistics were calculated using one-way ANOVA (B–D). The mean ± SEM is shown (B, D, and E). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001