Fig. 1. CB2R inhibition in combination with PE counteracts chronic stress-induced behavioural impairments.

a Schematic representation of the experimental design. b uCMS induces anxiety-like behaviour (decrease in time spent in open arms during EPM) that is ameliorated by AM630 treatment in combination with PE. c Increased latency to groom in the SST evoked by uCMS returns to similar levels as CTRL with AM630 treatment in combination with PE. d uCMS-induced immobile behaviour in FST is decreased in animals subjected to AM630 treatment in combination with PE. e Treatment with AM630 in combination with PE increases time spent in the centre zone of the OF test. f uCMS-induced cognitive impairments in NOR are ameliorated by PE alone or in combination with AM630 treatment. Data presented as mean ± SEM and circles represent individual data points (animals) [Student’s t test: ***p < 0.001, **p < 0.01 vs CTRL; Two-way ANOVA: ^##p < 0.01; ^#p < 0.05 vs uCMS; further statistical details in Table S4]. uCMS unpredictable chronic mild stress, AM630 CB2R inverse agonist, PE physical exercise, no PE no physical exercise.