TABLE 1.
Oligonucleotide primers used for mutagenesis
| Primer | Sequence |
|---|---|
| A | CCA CTA GTG TGC ACC CTT TCA AGT GAT |
| B | CCA CTA GTG AAA ATA GGG ACC AAA AC |
| C | ATT TAG GTG ACA CTA TA |
| D | CCG GGG ACC CTT AAT CCA ATG CTT GGC GTT GTC AAC TTG GGA GCA |
| E | CCG GGG ACC GGA GCA ACT TTT TTG ATG ATG GTT TGC GCA AAG ACT |
| F | CCG GGG ACC AAG AGT GAA AGA GCC TTG AAT CAA CAG ATA GAT ATG |
| G | CCA GAT CTT CGC TTC TTC TTC AAT TCA |
| H | CCA GAT CTG CTG CTG TGG TTG CG |
| I | CCA GAT CTT GCA CCC TTT CAA GTG AT |
| J | CCA GAT CTG CTT TTA AAC CAT GAA GG |
| K | CCA GAT CTG GAG CAA CTT TTT TGA TGA |
| L | CCA GAT CTA AGA GTG AAA GAG CCT TG |
| M | AAT ACG ACT CAC TAT AG |
| Na | TGG GAC CAA AAC AGA ATA GCC TTC ATG GTT TAA A |
The TAG used in oligonucleotide N to introduce a stop codon for the pGEMCT construct and in lieu of NS3 aa 156 is underlined.