TABLE 2.

NS3 mutants used in this study

NS3 mutant	Position of mutated amino acids	Amino acid sequence deletedb	Glycosylation	Transport to Golgi
NS3			+	+
pGEMHID1a	Δ118–126	AIIHTTLLV	−	+
pGEMHID2a	Δ118–136	AIIHTTLLVA AVVALLTSV	−	+
pGEMHID3	Δ127–136	AAVVALLTSV	−	+
pGEMHID4	Δ127–147	AAVVALLTSV CTLSSDMSAFV	−	+
pGEMHIID1	Δ153–162	KTEVPSWFKS	±	+
pGEMHIID2	Δ153–172	KTEVPSWFKS LNPMLGVVNL	±	+
pGEMHIID3	Δ153–182	KTEVPSWFKS LNPMLGVVNLGATFLMMVCA	+	+
pGEMHIID4a	Δ163–172	LNPMLGVVNL	+	+
pGEMHIID5a	Δ163–182	LNPMLGVVNL GATFLMMVCA	+	+
pGEMCT	Δ156–229	VPSWFKSLN PMLGVVNLGA TFLMMVCAKS ERALNQQIDM IKKEVMKKQS YNDAVRMSFT EFSSIPLDGF EM PLT	+	+
GSIc	63		+	+
GSIIc	150		−	+

^aIn these constructs, the deleted amino acids were replaced by the dipeptide RS. 

^bThe sequences that contribute to the two hydrophobic domains are underlined. 

^cIn these mutants, there is an N-to-S change at the indicated positions.