FIG. 3.

Subset distribution of HSA-expressing thymocytes. Thymocytes obtained from biopsy samples of implant 15 at the indicated times postinfection were costained for human CD4, CD8, and CD45, as well as murine HSA. APC, allophycocyanin; Red 613, streptavidin red 613; PE, phycoerythrin; FITC, fluorescein isothiocyanate. (A) Gating was performed on total live thymocytes. The percentage of each indicated subset is based on relative distribution in total live thymocytes. (B) Gates were set on live CD45⁺ (human) cells, and the CD4 and CD8 distribution of these CD45⁺ cells is shown. The percentage of each indicated subset is based on relative distribution in CD45⁺ thymocytes. (C) Gates were further narrowed to include only the CD45⁺/HSA⁺ subset. The profiles in panel C illustrate CD4 and CD8 distribution of these CD45⁺/HSA⁺ cells and demonstrate the subsets of human thymocytes expressing HIV genes. Percentages of CD45⁺/HSA⁺ cells are shown in each quadrant.