TABLE 2.

Comparative transduction of monocyte-derived macrophages and NTN2 neurons by Mo-MuLV and HIV-2 vectors^a

Cell type	Transduction
	Mo-MuLV vector LNL6-GFP	HIV-2 vector LACG
HeLa	(1.0 ± 0.6) × 106	(1.0 ± 0.8) × 106
MDM	<1 × 101	(5.4 ± 1.1) × 105
	Mo-MuLV vector LZRNL	HIV-2 vector L15.7
HeLa	(0.8 ± 0.3) × 106	(1.1 ± 0.2) × 106
NTN2	<1 × 101	(2.3 ± 1.7) × 105

^aValues are mean transducing units per milliliter ± standard deviation from three experiments. HeLa cells and third-replate NTN2 neurons were transduced with pretitered HIV-2 vector pL15.7 and Mo-MuLV vector LZRNL; monocyte-derived macrophages (MDM) were transduced with HIV-2 vector pLAGC, and an Mo-MuLV GFP-encoding vector (LNL6-GFP) and scored by epifluorescence microscopy at 48 h. No background X-Gal staining of untransduced NTN2 cells occurred, even if cells were stained for 1 week. Heat treatment (56°C, 40 min), leaving the VSV-G expression plasmid pHCMV-G out of the producer cell transfection, or addition of 10 μM zidovudine reduced transduction of macrophages and NTN2 neurons to <1/ml.