Figure 6.

SUMO influences mTOR complex formation in striatal neuronal cells.A, immunoprecipitation of mTOR with mTOR IgG or control IgG and Western blotting for endogenous Raptor, Rictor, or GβL from striatal control CRISPR- or SUMO1/2/3-depleted (SUMO1/2/3Δ) cells in AA-starved (Krebs) and stimulated with 3 mM leucine (+Leu). B, quantification of indicated protein interactions from A. Error bar represents mean ± SEM, ∗∗∗∗p < 0.001 by Student’s t test. AA, amino acid; GβL, G protein β-subunit–like protein; IgG, immunoglobulin G; mTOR, mechanistic target of rapamycin; Raptor, regulatory protein associated with mTOR; Rictor, rapamycin-insensitive companion of mTOR; SUMO, small ubiquitin–like modifier.