Figure 4.

Immunofluorescent staining of F-actin in aBMSCs seeding on membranes, indicating cell attachment after 12 h (A) and 24 h (B)—scale bar: 50 μm (n = 3). (C) Representative SEM images showing cell–membrane interaction after a 7-day seeding on the membranes (n = 3). (D) alarmarBlue Cell Proliferation results of 1, 3, 5, and 7 days indicate that GelMA/PCL-TCP promoted cell proliferation significantly higher than others (n = 4)—different lowercase letters denote statistical differences between groups. (E) Representative images of Micro-CT showing the in vitro bone mineralization nodules formation (n = 3). (F) Quantified bone volume analysis of aBMSCs after 21 days of osteogenic induction (n = 3).