Figure 5.

Alginate NPs associated with clinically isolated CF mucoid P. aeruginosa-produced biofilms. (A) Pearson’s coefficient was calculated for ALG and DXS NPs interacting with biofilms produced from isolated mucoid P. aeruginosa strains. N = 3 independent biofilms were tested, and at least N = 5 images were taken per biofilm, though co-occurrence data of 1.0 were considered artifacts of the image analysis software and were excluded. Colored asterisks below the significance lines denote outlier data points. Statistics are calculated using an unpaired student’s t-test, where * means p < 0.05, ** means p < 0.01, and **** means P < 0.0001. Note that the open circles are images shown in the next panel. (B) Confocal imaging of biofilms formed by strain 4305, portrayed as the maximum intensity throughout the entire z-stack of the biofilm, edited with ZEN software. Scale bar = 20 μm.