| time (min) | flow rate (mL/min) | % A | % B |
| 0.0 | 0.450 | 100 | 0 |
| 2.5 | 0.450 | 5 | 95 |
| 3.0 | 0.450 | 5 | 95 |
| 3.5 | 0.450 | 100 | 0 |
| 4.5 | 0.450 | 100 | 0 |
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