| time (min) | flow rate (mL/min) | % A | % B |
| 0.0 | 0.450 | 95 | 5 |
| 4.5 | 0.450 | 5 | 95 |
| 5.0 | 0.450 | 5 | 95 |
| 5.5 | 0.450 | 95 | 5 |
| 6.0 | 0.450 | 95 | 5 |
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