| time (min) | flow rate (mL/min) | % A | % B |
| 0.0 | 0.500 | 90 | 10 |
| 1.5 | 0.500 | 10 | 90 |
| 2.0 | 0.500 | 10 | 90 |
| 2.5 | 0.500 | 90 | 10 |
| 5.5 | 0.500 | 90 | 10 |
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