REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
A1R antibody	Abcam, UK	ab151523
A1R antibody	Abcam, UK	ab82477, RRID: AB_2049141
SREBP1c antibody	Abcam, UK	ab28481, RRID: AB_778069
SREBP2 antibody	Abclonal, China	A13049, RRID, AB_2759897
FASN antibody	Cell signaling, USA	#3180, RRID: AB_2100796
ACC antibody	Cell signaling, USA	#3676, RRID: AB_2219397
CPT1 antibody	Proteintech, USA	ag7202
CD36 antibody	Abcam, UK	ab133625, RRID, AB_2716564
F4/80 antibody	Servicebio, China	GB113373, RRID, AB_2938980
CD11B antibody	Boster, China	BM3925, RRID: AB_2832991
TGF-β antibody	Abclonal, China	A2124, RRID: AB_2764143
Smad2 antibody	Affinity, China	AF6449, RRID: AB_2835272
pSmad2 antibody	Affinity, China	AF3449, RRID: AB_2834844
PKAc antibody	Cell signaling, USA	#4782, RRID: AB_2170170
SCAP antibody	Cell signaling, USA	#13102, RRID: AB_2798121
SCAP antibody	Abmart, China	TD13713
SCAP antibody	SantaCruz, USA	SC-13553, RRID: AB_628237
S2P antibody	Abcam, UK	ab140594
S2P antibody	Affinity, USA	DF13911
S1P antibody	Abcam, UK	ab140592, RRID: AB_2811283
Insig1 antibody	Abcam, UK	ab70784, RRID: AB_1269181
SQSTM1 antibody	Abcam, UK	ab109012, RRID: AB_2810880
NF-κB P65 antibody	Proteintech, USA	66535–1, RRID: AB_2881898
Phospho-NF-κB p65 antibody	Cell signaling, USA	#3033s, RRID: AB_331284
α-SMA antibody	Cell signaling, USA	#19245, RRID: AB_2734835
IκBα antibody	Cell signaling, USA	#4814s, RRID: AB_390781
LAMIN B1 antibody	Cell signaling, USA	#13435, RRID: AB_2737428
β-TUBULIN antibody	Cell signaling, USA	#2146, RRID: AB_2210545
GAPDH antibody	Proteintech, USA	60004-1, RRID: AB_2107436
ATP1A1 antibody	PTM Bio, China	PTM-6271
Bacterial and virus strains
AAV8-TBG-A1R	This paper	N/A
AAV8-TGB-GFP	This paper	N/A
Biological samples
Human liver paraffin blocks	This paper	N/A
Mouse liver paraffin blocks	This paper	N/A
Chemicals, peptides, and recombinant proteins
CCPA	Aladdin, China	C170001
DPCPX	Sigma, USA	C101
CPA	Sigma, USA	C8301
H89	Selleck, China	S1582
DbcAMP	Selleck, China	S7858
Cycloheximide	Selleck, China	S7418
MG132	MCE, USA	HY-13259
Bafilomycin A1	MCE, USA	HY-100558
Timosaponin AIII	MCE, USA	HY-N0810
PMSF	Beyotime, China	ST506
Critical commercial assays
IL-1β ELISA Kit	Mlbio, China	ml301814
IL-6 ELISA Kit	Mlbio, China	ml063159
TNFα ELISA Kit	Mlbio, China	ml002095
Insulin ELISA Kit	Mlbio, China	ml001983V
PKA kinase activity kit	Enzo Life Sciences, USA	ADI-EKS-390A
cAMP Gi Kit	Cisbio, France	62a.m.9PEB
Hoechst	Beyotime, China	P0133
LysoTracker red	Beyotime, China	C1046
Golgi Staining Kit	Abcam, UK	ab139483
Mouse genotyping kit	Vazyme, China	PD101-01
FastKing gDNA Dispelling RT SuperMix	TIANGEN, China	KR118
qPCR SYBR Green Master Mix	Vazyme, China	Q131
RIPA Lysis Buffer	Beyotime, China	P0013B
Nuclear and Cytoplasmic Protein Extraction Kit	Beyotime, China	P0028
Triglycerides Assay Kit	Njjcbio, China	A110-1-1
Total cholesterol assay kit	Njjcbio, China	A111-1-1
Alanine aminotransferase Assay Kit	Njjcbio, China	C009-2-1
Aspartate aminotransferase Assay Kit	Njjcbio, China	C010-2-1
Alkaline phosphatase assay kit	Njjcbio, China	A059-2-2
Low-density lipoprotein cholesterol assay kit	Njjcbio, China	A113-1-1
High-density lipoprotein cholesterol assay kit	Njjcbio, China	A112-1-1
Nonesterified Free fatty acids assay kit	Njjcbio, China	A042-2-1
Oil Red O Stain Kit	Njjcbio, China	D027-1-2
Duolink In Situ Wash Buffers, Fluorescence	Sigma, USA	DUO82049
Duolink In Situ Detection Reagents Red	Sigma, USA	DUO92008
Duolink In Situ PLA Probe Anti-Rabbit PLUS	Sigma, USA	DUO92002
Duolink In Situ PLA Probe Anti-Mouse MINUS	Sigma, USA	DUO92004
Immunoprecipitation Kit	Invitrogen, USA	10007D
Membrane and Cytosol Protein Extraction Kit	Beyotime, China	P0033
EZ-press RNA purification kit	EZBio, USA	B0004DP
DMEM	Meilunbio, China	MA0212
DMEM/F-12	Meilunbio, China	MA0214
Deposited data
Human NAFLD expression profiling data	GEO portal	GSE89632
Human NAFLD transcriptomic data	GEO portal	GSE135251
Experimental models: Cell lines
AML12 cells	ATCC	CRL-2254
HepG2 cells	ATCC	HB-8065
Experimental models: Organisms/strains
C57BL/6 Adora1liver−/− mice	Shanghai Model Organisms Center Inc.	N/A
Hepatic ADORA1-OE C57BL/6 mice	This paper	N/A
C57BL/6 mice	Shanghai SLAC Laboratory Animal Co., Ltd	N/A
Oligonucleotides
Primes for mouse see Table S3	This paper	N/A
Software and algorithms
Prism, version 10	GraphPad	https://www.graphpad.com/
LAS X, version 3.5.7	Leica	https://www.leica.com/