Figure 2.

Fusion neoantigens bind to diverse HLAs and can be detected among eluted HLA-bound peptides

(A) Frequency of class I HLAs expressed by 24 FLC patients.

(B) Frequency of 16 class I HLA alleles predicted to bind fusion neoepitopes, as reported by AlleleFrequencies.net for US populations, separated by six major racial/ethnic groups.

(C) Representative data from the biochemical HLA binding assay for fusion neoantigens predicted for HLA-A∗68:02.

(D) Summary of biochemical binding assay results across all 16 HLAs. See also Figure S1C.

(E) Number of fusion neoepitopes predicted for each patient in the cohort, color coded by results of the biochemical binding assay.

(F) Fragmentation spectrum of the EIFDRYGEEV peptide eluted from the A∗68:02 K562 cell line. See also Figure S2.