Figure 3.

Functional responses and TCRs directed against A∗68:02-EIFDRYGEEV among FLC patient TILs and PBMCs

(A) Schematic for the experiments. Created using BioRender.

(B) Intracellular cytokine staining for IFNγ and TNFα of FLC-SJ1 TILs stimulated with the indicated fusion peptides or control reagents.

(C) UMAP plots highlighting expression of IFNG and SJ1-4 TCR in SJ1 TILs after stimulation with EIFDRYGEEV.

(D) Differential expression of select genes between TILs expressing SJ1-4 TCR vs. all others in the dataset. See also Table S3.

(E) A∗68:02-EIFDRYGEEV tetramer staining of SJ1 PBMCs after expansion (STAR Methods).

(F) Frequency of TCR clonotypes among tetramer-positive cells from (E).