FIG. 2.

Analysis of virus production by p24 assay, RT assay, and immunoblot. Virions were purified and analyzed as previously described (40). (A) In the p24 assay, bars represent averages from five replicates and error bars show standard deviations; error bars for TAA are too small to be seen. (B) In the RT assay, bars represent averages from triplicates and error bars show standard deviations; error bars for COS-7 and TAA are too small to be seen. (C and D) For immunoblots, viral lysates were separated by SDS-12% PAGE, transferred onto nitrocellulose filters, and blotted with an HIV-1-positive human serum. (D) Alternatively, lysates were blotted with a MAb against HIV-1 RT (purchased from Biotechnology Transfer, Inc. [Columbia, Md.]) or an antiserum to HIV-1 IN (catalog no. 757; obtained through the AIDS Research Reagent Program, Division of AIDS, National Institute of Allergy and Infectious Diseases). The arrow indicates the position of the p6-truncated Gag precursor molecule. Lane C, viral lysate from COS cells transfected with an HIV-1 protease-mutant virus which contains only unprocessed Pr55^Gag and Pr160^Gag-Pol precursors.