FIG. 4.
Intracellular and virion-associated viral proteins from transfected COS-7 cells. (A) Cell and viral lysates (left and right panels, respectively) were obtained from mock-, wild-type-, and LTALL-transfected COS-7 cells and were blotted with the human HIV-1-positive serum. (B) Viral lysates were obtained from mock-, wild-type-, and PTAP−-transfected COS-7 cells and blotted with the human HIV-1-positive serum (upper panel) or the MAb against HIV-1 RT (lower panel). (C) Viral lysates were obtained from mock-, PR−-, and PR−/PTAP−-transfected COS-7 cells and were blotted with the human HIV-1-positive serum (upper panel) or the MAb against HIV-1 RT (lower panel). The amounts of viral proteins (p24 equivalent) for PR− and PR−/PTAP− are indicated.