Figure 2. Increased HSPC proliferation triggered by estrogen in female mice is abolished upon IGF1R-loss.

(a) Percentages of Ki67⁺ cells were measured for both LSK and WBM populations to reflect cells in cell cycle. n≥5 for both male and female Vav-Cre;Igf1r^L/L and Vav-Cre mice. (b) Percentages of Ki67⁺ proliferating cells were measured for all LSK subpopulations. n≥5 for both male and female Vav-Cre;Igf1r^L/L and Vav-Cre mice. (c) Percentages of Ki67⁺ proliferating cells were measured for HSCs of female or male mice with the indicated genotypes (legend the same as in d) treated with oil (O) or β-estradiol (E), and are shown as fold changes in relation to those of corresponding controls (i.e., oil-treatment). n≥3 for both male and female Vav-Cre;Igf1r^L/L and Vav-Cre mice. (d) Percentages of AnnexinV⁺ apoptotic cells were measured for HPCs of female or male mice with the indicated genotypes treated with oil (O) or β-estradiol (E), and are shown as fold changes in relation to those of corresponding controls (i.e., oil-treatment). n≥3 for both male and female Vav-Cre;Igf1r^L/L and Vav-Cre mice. Data represent mean ± SEM, *p<0.05, **p<0.01, ***p<0.005, ****p<0.001, n.s.=not significant.