Table 1.
SARS-CoV-3 Reagents and Sources
| Reagent | Description | Source | Catalogue Number | Usage |
|---|---|---|---|---|
| TBS | Tris Buffered Saline with Casein | BioRad | 1610782 | DBS Elution |
| FLT | Full Length Trimeric SARS-CoV-2 Spike | MassBiologics | N/A | 8-Plex MIA/iACE2 |
| FLS | Full length SARS-CoV-2 Spike, His-Tag | Native Antigen | REC31868–100 | 8-Plex MIA |
| Spike Subunit 1 (S1) | SARS-CoV-2 (2019-nCoV) Spike S1, His-Tag | Sino Biological | 40591-V08H | 8-Plex MIA |
| RBD | SARS-CoV-2 Stable RBD, Thrombin-His | MassBiologics | N/A | 8-Plex MIA |
| Nucleocapsid (N) | 2019-nCoV Nucleocapsid Protein, His tag | SinoBiological | 40588-V08B | 8-Plex MIA |
| Nucleocapsid (N-NA) | SARS-CoV-2 Nucleoprotein, His-Tag (E. coli) | Native Antigen | REC31812–100 | 8-Plex MIA |
| Nucleocapsid (NHT) | SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag) | Sino Biological | 0588-V07E | 8-Plex MIA |
| Internal Control (IC) | Mouse anti-Human IgG3 Secondary Antibody | ThermoFisher | MA1–83242 | 8-Plex MIA |
| WT sRBD | SARS (WA1/2020) RBD, Thrombin-His | MassBiologics | N/A | iACE2 |
| hACE2 | Biotinylated ACE2 | Wadsworth Center Protein Core | N/A | iACE2 |
| CC12.3 | Human Mab RBD-A WT D614G | Scripps | N/A | iACE2/RVP |
| RVP-702L | WT D614G Strain Reporter Virus Particles | Integral Molecular | CL-275A | RVP |
| 293-hsACE2 Cells | 293T cells expressing human ACE2 receptor | Integral Molecular | TA-060520-MC | RVP |