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[Preprint]. 2024 Dec 29:2024.03.19.585778. Originally published 2024 Mar 20. [Version 2] doi: 10.1101/2024.03.19.585778

Figure 3. Assays of siRNA knockdown on osteoblast and adipocyte maturation and function.

Figure 3

Results of Alkaline phosphatase (ALP) assay in hFOBs (top panels), ALP assay in hMSC-Osteoblasts (second from top), alizarin Red S (ARS) assay in hMSC-Osteoblasts (third from top), and adipogenesis assay in hMSCs (bottom) separated by (A) uncorrected stain intensity, (B) cell counts, and (C) per-cell stain intensity. In each plot siRNA targets are listed along the x-axis. hMSC-based assays were measured and normalized in the treated state (with BMP2 or adipogenic induction media). Any siRNAs resulting in significant decreases of assayed measurements are displayed in blue and siRNAs with < 3 replicates were untested and are marked in charcoal.