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| Substrates | Vmax (pmol min−1) | Km (nM) | kcat (s−1)* | kcat/Km (pM−1 s−1) | |
|---|---|---|---|---|---|
| h-TREX1 | U5 Unprocessed | 20.21 ± 4.4 | 98.5 ± 15.2 | 10.2 ± 0.8 | 103.1 ± 0.6 |
| U5 Processed | 12.2 ± 3.2 | 54.2 ± 10.3 | 3.8 ± 0.3 | 70.2 ± 0.74 | |
| m-TREX1 | U5 Unprocessed | 39.8 ± 4.3 | 109.5 ± 15.7 | 9.5 ± 0.2 | 86.7 ± 0.62 |
| U5 Processed | 18.6 ± 6.4 | 90.6 ± 12.5 | 4.1 ± 0.32 | 45.2 ± 0.43 |
