Figure 7. MPER+ Sera Antibody Binding Profile.

Vaccine-matched MPER-peptide was screened for binding longitudinal sera Abs from HVTN133 vaccine (A) or placebo (B) recipients via ELISA. Arrows indicate immunization timepoints. Each symbol represents an individual. Sera Abs post 2^nd and 3^rd immunizations from vaccine (C) and placebo (D) recipients were tested in ELISA for binding wild-type and MPER-bnAb epitope knockout-mutant. (E) Sera Ab binding to single-alanine scanned SP62 peptides containing the proximal MPER-bnAb epitope. Binding responses is shown relative to the wild-type peptide. Bars represent the geometric mean. (F) Binding titers measured in Log AUC for sera Abs to wild-type and mutant SP62 peptides described in panel E. Asterisks in key indicate the individuals with less than 50% binding to the ⁶⁶³LDKW⁶⁶⁶ mutant peptides relative to the wild-type. (G) Sera Ab binding to kynureninase via ELISA. Binding titer is reported as Log AUC. For all ELISAs, binding titers reported were background subtracted.