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. 2024 Mar 25;15(1):2333237. doi: 10.1080/21505594.2024.2333237

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 4. — The characteristics of lncRNA53557 in DT40 cells. (a) The coding potential of lncRNA53557 was analysed by the PhyloCSF analysis. ACTB served as coding RNA control, and MALAT1 and XIST served as noncoding RNA controls. The regions with a score less than 0 (below the baseline) were predicted to be noncoding, while regions with a score greater than 0 (above the baseline) were predicted to be coding. (b and c) the coding potential of lncRNA53557 was analysed by CPC and CPAT. ACTB and GAPDH served as coding RNA controls, and MALAT1 and XIST served as noncoding RNA controls. (d) Subcellular fractionation experiment to analysed the localization of lncRNA53557 in DT40 cells. GAPDH and U6 were used as cytoplasmic and intranuclear reference, respectively. Data are shown as the mean ± SD for six independent experiments.