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. 2024 Mar 25;15(1):2333237. doi: 10.1080/21505594.2024.2333237

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 5. — LncRNA53557 upregulated STAT1 expression and inhibited vvIBDV replication. (a) Relative expression levels of lncRNA5357 at various time points was measured in IBDV-infected DT40 cells by RT-qPCR. (b) Verification of the overexpression of the lncRNA53557 by RT-qPCR. (c) The effect of transfection on cell viability. (d) Relative mRNA and protein expression of STAT1 were determined by RT-qPCR and Western Blotting in lncRNA5357 overexpressed DT40 cells infected with vvIBDV. (e) Expression of vvIBDV-VP2 gene and protein were determined by RT-qPCR and Western Blotting in lncRNA5357 overexpressed DT40 cells infected with vvIBDV. (f) Relative mRNA and protein expression of STAT1 were determined by RT-qPCR and Western Blotting in lncRNA5357 knocked-down DT40 cells infected with vvIBDV. (g) Expression of vvIBDV-VP2 gene and protein were determined by RT-qPCR and Western Blotting in lncRNA5357 knocked-down DT40 cells infected with vvIBDV. Data are shown as the mean ± SD for three independent experiments. *p < 0.05, **p < 0.01.