Figure 8.

gga-miR-3530-5p directly targets STAT1 3’UTR. (a) Target relationship between gga-miR-3530-5p and STAT1 was predicted by TargetScan. (b) Schematic illustration of STAT1-WT and STAT1-mut luciferase reporter vectors. (c) Luciferase activities were detected in HEK-293T or DT40 cells co-transfected with gga-miR-3530-5p and luciferase reporters containing nothing, STAT1, or mutant transcript. Relative firefly luciferase expression was normalized to renilla luciferase. Data are shown as the mean ± SD for three independent experiments. *p < 0.05, ** or ##P < 0.01.