Figure 10.

LncRNA53557 serves as a miRNA sponge of gga-miR-3530-5p to regulate STAT1 expression. (a) Luciferase activities were evaluated in HEK-293T or DT40 cells transfected with luciferase reporters containing STAT1 3’ UTR, mut or nothing. Data are presented as the relative ratio of firefly to renilla luciferase activity. (b) Relative mRNA and protein levels of STAT1 were measured by RT-qPCR and Western Blotting in DT40 cells co-transfected with pcDNA3.1 and NC or gga-miR-3530-5p mimics, or co-transfected with pcDNA3.1-lncRNA53557 and NC or gga-miR-3530-5p mimics. (c) Relative mRNA levels of Mx1, OASL, and ISG15 was measured by RT-qPCR and Western Blotting in DT40 cells co-transfected with pcDNA3.1 and NC or gga-miR-3530-5p mimics, or co-transfected with pcDNA3.1-lncRNA53557 and NC or gga-miR-3530-5p mimics. Data are shown as the mean ± SD for three independent experiments. *p < 0.05, **p < 0.01.