Table 1.
Names | Primer sequences (5’-3’) |
---|---|
Wild-type (WT) and mutant-type (Mut) primers of STAT1 3’UTR | |
WT Forward | AGCTTGTATCTCGGATGGTGGATCCAGCAGAGATTGACACAGTAATGTGTTCAGAGCT |
WT Reverse | CTGAACACATTACTGTGTCAATCTCTGCTGGATCCACCATCCGAGATACA |
Mut Forward | AGCTTGTATCTCGGATGGTGGATCCATTAGCTATTGACACAGTAATGTGTTCAGAGCT |
Mut Reverse | CTGAACACATTACTGTGTCAATAGCTAATGGATCCACCATCCGAGATACA |
Wild-type (WT) and mutant-type (Mut) primers of lncRNA53557 | |
WT Forward | AGCTTGGAAATATTCTGTGTCTGCTCAGAGCAGAGCCTCCAGCCTGAGTCTCCCAGAGCT |
WT Reverse | CTGGGAGACTCAGGCTGGAGGCTCTGCTCTGAGCAGACACAGAATATTTCCA |
Mut Forward | AGCTTGGAAATATTCTGTGTCTGCTCAGATTATCGCCTCCAGCCTGAGTCTCCCAGAGCT |
Mut Reverse | CTGGGAGACTCAGGCTGGAGGCGATAATCTGAGCAGACACAGAATATTTCCA |
Note: Four sequences inserted in the pMIR-REPORT plasmids. The red colour indicates wild-type bases (for the target location), and green indicates the mutated bases.