Table 12. Percentage of live and dead cells from different cell lines treated with I. coccinea leaf ethyl acetate extract.
SD: standard deviation; IC50: half-maximal inhibitory concentration; SEM: standard error of mean; HaCaT cell line: keratinocyte cell line
Values are means of triplicate experiments
Cell line used | HaCaT cell line | Human dermal fibroblasts | A375 cell lines (malignant melanoma cell lines) | |||||||||
Concentration of extract (µg/mL) | % cell death mean | SD | SEM | % live cells | % cell death | SD | SEM | % live cells | % cell death | SD | SEM | % live cells |
100 | 48.98 | 1.39 | 0.80 | 51.02 | 63.98 | 0.56 | 0.32 | 36.02 | 86.30 | 7.95 | 4.59 | 13.70 |
50 | 44.97 | 0.98 | 0.56 | 55.03 | 49.77 | 2.17 | 1.25 | 50.23 | 84.24 | 1.27 | 0.73 | 15.76 |
25 | 39.18 | 1.74 | 1.00 | 60.82 | 41.19 | 0.66 | 0.38 | 58.81 | 66.34 | 7.29 | 4.21 | 33.66 |
12.5 | 30.48 | 1.42 | 0.82 | 69.52 | 32.58 | 0.58 | 0.34 | 67.42 | 59.98 | 5.94 | 3.43 | 40.02 |
6.25 | 21.84 | 1.27 | 0.74 | 78.16 | 29.97 | 0.56 | 0.32 | 70.03 | 53.55 | 4.69 | 2.71 | 46.45 |
3.125 | 21.22 | 1.01 | 0.58 | 78.78 | 27.63 | 0.65 | 0.38 | 72.37 | 37.54 | 2.40 | 1.39 | 62.46 |
1.5 | 12.38 | 1.58 | 0.91 | 87.62 | 15.42 | 4.12 | 2.38 | 84.58 | 24.67 | 3.57 | 2.06 | 75.33 |
Control | 100 | 100.00 | 100 | |||||||||
IC50 | 98.52 µg/mL | IC50 | 58.52 µg/mL | IC50 | 7.96 µg/mL |