Key resources table
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Bacterial and virus strains | ||
AAV5-CAMKIIa-GCaMP6f-WPRE-SV40 | Penn Vector Core | N/A |
AAV8-CAMKIIa-ChRmine-mScarlet-Kv2.1-WPRE | Stanford Vector Core | GVVC-AAV-194 |
Chemicals, peptides, and recombinant proteins | ||
VECTASHIELD Hardset Antifade Mounting Medium with DAPI | Vector Laboratories | CAT#H-1800 |
Sucrose, 99% | Fisher Scientific | AAA155830C |
Quinine hydrocholoride dihydrate | Sigma-Aldrich | Q1125 |
Experimental models: Organisms/strains | ||
C57BL/6J | Jackson Laboratory | 000664 |
Software and algorithms | ||
FIJI/ImageJ | NIH | https://fiji.sc/ |
Prairie View | Bruker | N/A |
MATLAB | Mathworks | https://www.mathworks.com/products.html |
Python 3.0 | Python Software Foundation | https://www.python.org/ |
SIMA | 46 | http://www.losonczylab.org/sima/1.3.2/ |
NAPE Calcium imaging pipeline | Github/Zenodo | https://doi.org/10.5281/zenodo.10076964 |
PRISM 8 | GraphPad | https://www.graphpad.com/ |
Illustrator CS8 | Adobe | https://www.adobe.com/products/illustrator.html |
Other | ||
ProView GRIN lens (0.6mm x 7.3mm) | Inscopix | 1050-004608 |
Ultima 2P Plus w/ NeuraLight 3D Spatial Light Modulator | Bruker | https://www.bruker.com/en/products-and-solutions/fluorescence-microscopy/multiphoton-microscopes/ultima-2pplus.html |
Insight X3 Tunable Ultrafast Laser | Spectra-Physics | https://www.spectra-physics.com/en/f/insight-x3-tunable-laser |
Spirit High Power Femtosecond Lasers | Spectra-Physics | https://www.spectra-physics.com/en/f/spirit-femtosecond-laser |