Fig. 3.

Sequence alignment of ECD1 domains of ABCA1 and ABCA7. Amino acid sequences for the corresponding ECD1s were obtained from UniProt.org (ABCA1 ID: O95477, residues 43–639; ABCA7 ID: Q8IZY2, residues 43–549). The numbering of the alignment starts with the beginning of the ECD1. The location of the missense variants that confer AD risk in African American/Black adults (T319A, H395R) and the missense variant predicted to have a protective effect (G215S) are indicated by stars. Intramolecular disulfide bonds implicated in apoA-I-dependent cholesterol efflux are denoted by circles. The Trp residue at position 590 linked to Tangier disease in ABCA1 is conserved in ABCA7 and highlighted by a triangle. Sequences were aligned using the Tcoffee multiple sequence alignment in Jalview [112]. The alignment was color coded using the ClustalX scheme. Colors represent categories of amino acids; white indicates gap/unconserved residues.