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. 2024 Feb 12;96(7):3077–3086. doi: 10.1021/acs.analchem.3c05194

Table 1. Estimated isoAsp Formation at Each isoAsp Site in Aged Rituximaba.

    fraction of Asn/Asp/isoAsp residues (%)
position peptide sequence Asn conversion to Asp conversion to isoASP
Rituximab_Light chain
Asn52 ATSNLASGVPVRF 99.8 n.o. 0.2
Asn157 KVDNALQSGNSQESVTEQDSKDSTY 94.4 1.2 4.4
Rituximab_Heavy chain
Asn55 IGAIYPGNGDTSY 69.3 4.4 26.3
Asn163 SWNSGALTSGVH 69.7 4.0 26.3
Asn319 TVLHQDWLNGKEY 97.2 n.o. 2.8
a

n.o.: Not observed. The amino acid residues converted to isoAsp are underlined.