Table 1. Estimated isoAsp Formation at Each isoAsp Site in Aged Rituximaba.
| fraction
of Asn/Asp/isoAsp residues (%) |
||||
|---|---|---|---|---|
| position | peptide sequence | Asn | conversion to Asp | conversion to isoASP |
| Rituximab_Light chain | ||||
| Asn52 | ATSNLASGVPVRF | 99.8 | n.o. | 0.2 |
| Asn157 | KVDNALQSGNSQESVTEQDSKDSTY | 94.4 | 1.2 | 4.4 |
| Rituximab_Heavy chain | ||||
| Asn55 | IGAIYPGNGDTSY | 69.3 | 4.4 | 26.3 |
| Asn163 | SWNSGALTSGVH | 69.7 | 4.0 | 26.3 |
| Asn319 | TVLHQDWLNGKEY | 97.2 | n.o. | 2.8 |
n.o.: Not observed. The amino acid residues converted to isoAsp are underlined.