FIGURE 4.

Liver biopsy stains for PD-1/PD-L1 across patient cohorts with chronic hepatitis B. (A) Area of PD-1+ expression among intrahepatic immune cells across chronic hepatitis B cohorts. (B) Area of PD-L1+ expression among intrahepatic immune cells across chronic hepatitis B cohorts. (C) Correlation between PD-L1+ area among intrahepatic immune cells and PD-L1 scoring on hepatocytes among IC patients. (D) Representative PD-L1 staining on hepatocytes to demonstrate weak (left) and diffuse (right) stains, black scale bars represent specified lengths. Unpaired parametric t tests were conducted to compare marker expression between cohorts (A, B). Pearson correlation coefficient and p-values are listed (C). (*p<0.05). Abbreviations: IA, immune-active; IC, inactive carrier; IT, immunotolerant; PD-1, programmed cell death protein 1; PD-L1, programmed cell death ligand 1; Tx, treatment.