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Flow cytometry panel for CAR transduction efficiency using 5-laser Cytek Aurora.
| Antigen | Clone | Fluor | Channel | Company | Catalogue # |
|---|---|---|---|---|---|
| Fixable viability dye | “Blue” | UV6 | Thermo Fisher | L23105 | |
| Flag Tag | M2 | APC | R1 | Abcam | AD0059F |
| CD8 | 53–6.7 | PEcy7 | YG9 | Invitrogen | 53–6.7 |
| CD4 | RM4–5 | Pac blue | V3 | Biolegend | 100531 |
| CD69 | H1.2F3 | BV650 | V11 | Biolegend | 104541 |
