Figure 2 .

Role of ATIP/ATIP1 in tumor cell motility

PC3 cells transfected with NTC siRNA or ATIP siRNA were reconstituted with ATIP1 or ATIP3 and then used for the subsequent analyses. (A,B) PC3 cells transfected as indicated were analyzed for migration across Transwell inserts. Representative images of DAPI-stained nuclei of migrated cells are shown (A), and the number of migrated cells was quantified for each condition (B). Scale bar: 200 μm. (C,D) PC3 cells transfected as indicated were analyzed for cell invasion across Matrigel-coated Transwell inserts. Representative images of DAPI-stained nuclei of invaded cells are shown (C), and the number of invaded cells was quantified for each condition (D). Scale bar: 200 μm. (E,F) PC3 cells transfected as indicated were analyzed for directional cell migration in a wound closure assay (E), and the area covered by cell migration was quantified at indicated time point (F). Scale bar: 200 μm. (G) PC3 cells transfected as indicated were analyzed by western blot analysis. P indicates phosphorylation. NTC siRNA, non-targeting control siRNA. ***P<0.0001, N.S., not significant.