Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Apr 1;14:7632. doi: 10.1038/s41598-024-58004-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

CircGPC3 regulates HCC growth and metastasis by sponging miR-578. (A) Venn diagrams showing overlapping miRNAs that bind to circGPC3 identified by circBank and circInteractome. (B) CircGPC3 biotin-labeled probes could capture more circGPC3. (C, D) In the RNA pull-down assay, qRT‒PCR analysis was performed on PLC/PRF/5 and Hep3B2.1-7 cells to predict circGPC3-target miRNAs. (D) Schematic illustration of the binding sites of circGPC3 and miR-578. (E–G) Relative luciferase activities were calculated in PLC/PRF/5 and Hep3B2.1-7 cells cotransfected with miR-578 mimics or mimic-NC and circGPC3-WT or circGPC3-MUT vectors. (H) FISH assay was used to observe the subcellular localization of circGPC3 (red) and miR-578 (green). (I) qRT‒PCR was performed to evaluate the expression of miR-578 in HCC and adjacent normal tissues. (J, K) Transwell assays were used to evaluate the migratory and invasive capabilities of PLC/PRF/5 and Hep3B2.1-7 cells by transfecting these cells with miR-578 mimics or inhibitors. (L, M) Relative migration and invasion rates of PLC/PRF/5 and Hep3B2.1-7 cells were calculated for cells transfected with miR-578 mimics or inhibitors. (N) EdU assays were used to evaluate the proliferative capacities of PLC/PRF/5 cells after transfection with miR-578 mimics or inhibitors. **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant.