Table 2.
Oligonucleotides used to generate the exoYC KO mutant
| Primer | Sequence | Reference | |
|---|---|---|---|
| 1 | exoYC KO upstream forward primer with homology to pCR™8/GW/TOPO™ |
ACAAAAAAGCAGGCTCCGAATTCGCC CTTAATCACGGCAGTGACATCCGCC |
This study |
| 2 | exoYC KO upstream reverse primer with homology to gentamycin cassette |
CAGTTTACGAACCGAACAGGCTTATGT CAAATTGGCAACACCCGTCCCAATGAGG |
This study |
| 3 | exoYC KO downstream forward primer with homology to gentamycin cassette |
GCACTTTGATATCGACCCAAGTACCGCC ACCTAACCGTCCTGGGCTAACTGTGATGG |
This study |
| 4 | exoYC KO downstream reverse primer with homology to pCR™II-TOPO™ |
CTTTGTACAAGAAAGCTGGGTCGAATTCG CCCTCACCATGTCATCGATCACACTGGCG |
This study |
| 5 | exoYC verification forward primer | CGCAGGCACTGTGATGGTCC | This study |
| 6 | exoYC verification reverse primer | CGTCGATCAGCTTCTCACAG | This study |
| 7 | Gentamycin cassette forward primer | GTTGACATAAGCCTGTTCG | 28 |
| 8 | Gentamycin cassette reverse primer | GTTAGGTGGCGGTACTTGG | 28 |
| 9 | Gentamycin cassette verification forward primer | GTGCAAGCAGATTACGGTGACG | 28 |
| 10 | Gentamycin cassette verification reverse primer | GAGCCTACATGTGCGAATGATGC | 28 |