Table 3.

Results obtained by using methylome sequencing methods (multimodal omics sequencing methods) and categories of those findings.

Categories	Method	Results	Ref
Development	scCOOL-seq	Chromatin conformation and DNA methylation undergo distinct changes that do not happen simultaneously following fertilization	74
	scNOMeRe-seq	Role of DNA methylation remodeling in reconstructing genetic lineages in early embryos	86
	scTrio-seq2	Slower genome demethylation in primitive endoderm cells than in epiblast and trophectoderm cells	71
	sn-m3C-seq	Remodeling of DNA methylation is temporally separated from chromatin state dynamics and mainly occurs during late-gestational to early-infant-development (In human frontal cortex and hippocampus)	65
	iscCOOL-seq	Integrating and analyzing of chromatin accessibility, DNA methylation and gene expression in growing mouth oocytes	73
	scM&T-seq	Correlations of methylation patterns of distal regulatory regions with gene expression (pluripotency factors)	60
	scNMT-seq	De novo methyltransferase is dispensable for major cell type development at E8.5 in mouse development but crucial for silencing prior or alternative cell fates such as pluripotency and extraembryonic programs	98
Brain	scMT-seq	Negative correlation between methylation in non-CGI promoters and gene expression in dorsal root ganglion neurons	61
	snmCAT-seq	Reconstruction of regulatory lineages for cortical cell populations Identification of distinct genetic risk enrichment associated with neuropsychiatric traits	87
	sn-m3C-seq	Constructing single-cell DNA methylome and 3D genome structure atlas of adult mouse brain	66
Senescence	scM&T-seq	Relation of aging with a global increase in transcription and methylation heterogeneity	99
Tumor	scTrio-seq	Identification of subpopulation of human hepatocellular carcinoma cells and cellular heterogeneity within a subpopulation	69
	scTrio-seq2	Differences in DNA methylation levels between primary and metastatic colorectal tumors are mainly caused by the different sublineage composition	70