Table 1.
Immunophenotyping panel for multiplexed tissue imaging of cancer.
| Antigen | Clone | Dilution | Order No. | Fluorochrome (+ tested alternatives) | Company |
|---|---|---|---|---|---|
| Actin (smooth muscle) | REAL650 | 50 | 130-123-363 | FITC | Miltenyi Biotec |
| AFP | C3 | 50 | sc-8399PE | PE | Santa Cruz Biotechnology |
| B7-H4 | MIH43 | 100 | 358104 | PE | BioLegend |
| Bcl-2 | REA872 | 50 | 130-114-230 | FITC | Miltenyi Biotec |
| Bcl-xL | H-5 | 200 | Sc-8392PE | PE | Santa Cruz Biotechnology |
| CCL18 | REA487 | 50 | 130-107-608 | PE | Miltenyi Biotec |
| CD10 | REAL318 | 50 | 130-118-368 | FITC | Miltenyi Biotec |
| CD105 | REA794 | 50 | 130-112-169 | FITC | Miltenyi Biotec |
| CD107a | REA792 | 50 | 130-111-620 | FITC | Miltenyi Biotec |
| CD112 | REA1195 | 50 | 130-122-770 | PE | Miltenyi Biotec |
| CD117 | REA787 | 50 | 130-111-592 | PE (APC) | Miltenyi Biotec |
| CD11a | REA378 | 50 | 130-124-886 | FITC | Miltenyi Biotec |
| CD11b | REA713 | 50 | 130-110-552 | FITC | Miltenyi Biotec |
| CD11c | REA618 | 50 | 130-113-587 | PE | Miltenyi Biotec |
| CD123 | REA918 | 50 | 130-115-263 | FITC (APC) | Miltenyi Biotec |
| CD133 | REA820 | 50 | 130-112-195 | PE | Miltenyi Biotec |
| CD138 | REA929 | 50 | 130-115-479 | PE (APC) | Miltenyi Biotec |
| CD146 | REA773 | 50 | 130-111-322 | PE | Miltenyi Biotec |
| CD15 | VIMC6 | 50 | 130-113-484 | FITC | Miltenyi Biotec |
| CD155 | REA1081 | 50 | 130-118-998 | PE | Miltenyi Biotec |
| CD163 | REA812 | 50 | 130-112-132 | FITC | Miltenyi Biotec |
| CD169 | REA1176 | 50 | 130-121-106 | PE | Miltenyi Biotec |
| CD171 | L1-OV198.5 | 200 | 371604 | PE | BioLegend |
| CD183 | REA232 | 50 | 130-120-452 | PE | Miltenyi Biotec |
| CD184 | REA649 | 50 | 130-117-504 | PE | Miltenyi Biotec |
| CD19 | LT19 | 50 | 130-113-168 | FITC | Miltenyi Biotec |
| CD196 | REA190 | 50 | 130-120-458 | PE | Miltenyi Biotec |
| CD1c | AD5-8E7 | 50 | 130-113-301 | FITC | Miltenyi Biotec |
| CD2 | REA1130 | 50 | 130-119-508 | PE | Miltenyi Biotec |
| CD20 | REA1087 | 50 | 130-118-292 | FITC (PE) | Miltenyi Biotec |
| CD200 | REA1067 | 50 | 130-118-128 | FITC | Miltenyi Biotec |
| CD206 | DCN228 | 50 | 130-123-671 | FITC (PE) | Miltenyi Biotec |
| CD21 | REA940 | 50 | 130-115-609 | FITC | Miltenyi Biotec |
| CD22 | REA340 | 50 | 130-124-223 | FITC | Miltenyi Biotec |
| CD24 | REA832 | 50 | 130-112-656 | PE | Miltenyi Biotec |
| CD243 | REA495 | 50 | 130-124-440 | PE (APC) | Miltenyi Biotec |
| CD25 | REA945 | 50 | 130-115-534 | PE (APC) | Miltenyi Biotec |
| CD25 | M-A251 | 50 | 356104 | PE | BioLegend |
| CD26 | FR10-11G9 | 50 | 130-126-362 | PE | Miltenyi Biotec |
| CD27 | REA499 | 50 | 130-113-639 | FITC (PE) | Miltenyi Biotec |
| CD276 | REA1094 | 50 | 130-118-570 | PE (FITC) | Miltenyi Biotec |
| CD3 | REA1151 | 50 | 130-120-267 | FITC (APC) | Miltenyi Biotec |
| CD31 | REA1028 | 50 | 130-117-224 | FITC | Miltenyi Biotec |
| CD314 | REA1228 | 50 | 130-124-341 | PE | Miltenyi Biotec |
| CD34 | REA1164 | 50 | 130-120-515 | PE | Miltenyi Biotec |
| CD36 | REA760 | 50 | 130-110-739 | FITC | Miltenyi Biotec |
| CD38 | REA671 | 50 | 130-117-717 | PE (APC) | Miltenyi Biotec |
| CD39 | REA739 | 50 | 130-110-650 | PE | Miltenyi Biotec |
| CD4 | REA623 | 50 | 130-114-531 | FITC (PE) | Miltenyi Biotec |
| CD40 | REA733 | 50 | 130-110-946 | PE | Miltenyi Biotec |
| CD44 | REA690 | 50 | 130-113-342 | PE (FITC) | Miltenyi Biotec |
| CD45 | REA747 | 50 | 130-110-632 | PE | Miltenyi Biotec |
| CD45RA | T6D11 | 50 | 130-113-355 | FITC | Miltenyi Biotec |
| CD45RO | UCHL1 | 50 | 130-113-549 | FITC | Miltenyi Biotec |
| CD47 | REA220 | 50 | 130-123-754 | PE | Miltenyi Biotec |
| CD48 | REA426 | 50 | 130-106-516 | PE | Miltenyi Biotec |
| CD49α | REA1106 | 11 | 130-119-305 | FITC | Miltenyi Biotec |
| CD49β | REA188 | 11 | 130-100-337 | FITC | Miltenyi Biotec |
| CD54 | REA266 | 50 | 130-120-711 | PE | Miltenyi Biotec |
| CD56 | AF12-7H3 | 50 | 130-113-307 | PE (APC) | Miltenyi Biotec |
| CD57 | REA769 | 50 | 130-111-810 | PE | Miltenyi Biotec |
| CD61 | REA761 | 50 | 130-110-748 | FITC | Miltenyi Biotec |
| CD64 | REA987 | 50 | 130-116-195 | FITC | Miltenyi Biotec |
| CD66b | REA306 | 50 | 130-123-694 | FITC | Miltenyi Biotec |
| CD69 | FN50 | 50 | 130-113-524 | FITC (PE) | Miltenyi Biotec |
| CD70 | REA292 | 100 | 130-104-307 | FITC | Miltenyi Biotec |
| CD71 | REA902 | 50 | 130-115-028 | FITC (PE) | Miltenyi Biotec |
| CD73 | REA804 | 50 | 130-111-908 | PE (APC) | Miltenyi Biotec |
| CD74 | REA1103 | 50 | 130-119-026 | PE | Miltenyi Biotec |
| CD8 | REA734 | 50 | 130-110-677 | FITC | Miltenyi Biotec |
| CD9 | REA1071 | 50 | 130-118-806 | FITC | Miltenyi Biotec |
| CD90 | REA897 | 50 | 130-114-859 | FITC | Miltenyi Biotec |
| CD95 | REA738 | 50 | 130-113-004 | PE (FITC) | Miltenyi Biotec |
| CD96 | REA195 | 100 | 130-101-032 | PE | Miltenyi Biotec |
| CD99 | REA1174 | 50 | 130-121-078 | PE | Miltenyi Biotec |
| Collagen III | REAL912 | 50 | 130-127-357 | PE | Miltenyi Biotec |
| Collagen IV | REAL567 | 50 | 130-122-866 | PE | Miltenyi Biotec |
| CSF1R | 12-3A3-1B10 | 100 | NBP1-43362PE | PE | Novus Biologicals |
| CTLA-4 | BNI3 | 200 | 369604 | PE | BioLegend |
| Desmin | REA1134 | 50 | 130-119-489 | FITC (APC, PE) | Miltenyi Biotec |
| EpCAM | REA764 | 50 | 130-110-998 | FITC | Miltenyi Biotec |
| Fibronectin | REAL555 | 50 | 130-122-864 | PE | Miltenyi Biotec |
| FOLR2 | NBP2-99741 | 100 | NBP2-99741F | FITC | Novus Biologicals |
| FoxP3 | 236A/E7 | 50 | 12-4777-42 | PE | Thermo Fisher Scientific |
| FSP-1 | NBP2-36431 | 200 | NBP2-36431F | FITC | Novus Biologicals |
| Galectin 9 | REA435 | 50 | 130-124-237 | PE (APC) | Miltenyi Biotec |
| GD2 | 14.G2a | 50 | 562100 | PE (FITC) | BD |
| GFAP | REA335 | 50 | 130-118-351 | PE | Miltenyi Biotec |
| Glypican 3 | 307801 | 200 | FAB2119G | FITC | RnD Systems |
| H2AX | REA502 | 50 | 130-125-883 | PE | Miltenyi Biotec |
| HER2 | REA1232 | 50 | 130-124-466 | PE | Miltenyi Biotec |
| HIF-1 | EP1215Y | 200 | ab190197 | FITC | Abcam |
| HLA-ABC | REA230 | 50 | 130-120-055 | PE (APC, FITC) | Miltenyi Biotec |
| HLA-DR | REA805 | 50 | 130-111-789 | PE (APC, FITC) | Miltenyi Biotec |
| HLA-DR/DP/DQ | REA332 | 50 | 130-120-715 | PE | Miltenyi Biotec |
| HNF-4α | H-1 | 50 | sc-374229PE | PE | Santa Cruz Biotechnology |
| Hsp70 | REA349 | 50 | 130-124-694 | FITC | Miltenyi Biotec |
| IDO | D5J4E | 100 | 103125 | PE | Cell Signaling Technology |
| Ki-67 | REA183 | 50 | 130-117-691 | FITC | Miltenyi Biotec |
| LAG3 | REA351 | 25 | 130-120-470 | PE | Miltenyi Biotec |
| MUC1 | 16A | 50 | 355604 | PE | BioLegend |
| Myosin | REA1107 | 100 | 130-119-313 | FITC | Miltenyi Biotec |
| NANOG | REA314 | 50 | 130-117-377 | PE | Miltenyi Biotec |
| p16 | F-12 | 50 | sc-1661PE | PE | Santa Cruz Biotechnology |
| p21 | F-8 | 50 | sc-271610PE | PE | Santa Cruz Biotechnology |
| p53 | REA1132 | 50 | 130-119-502 | PE | Miltenyi Biotec |
| Pan-Cytokeratin | REA831 | 50 | 130-112-743 | FITC (APC, PE) | Miltenyi Biotec |
| PD-1 | REA1165 | 50 | 130-120-382 | PE | Miltenyi Biotec |
| PDGFR β | MAB1263 | 200 | FAB1263T-100UG | FITC | RnD Systems |
| PD-L1 1 | REA1197 | 50 | 130-122-809 | PE | Miltenyi Biotec |
| PD-L1 2 | MIH2 | 50 | 393608 | PE | BioLegend |
| Podoplanin | REAL468 | 50 | 130-125-009 | FITC | Miltenyi Biotec |
| PSMA | GCP-04 | 100 | NBP1-45057AF488 | FITC | Novus Biologicals |
| S100A8 | REA917 | 50 | 130-115-253 | FITC (APC, PE) | Miltenyi Biotec |
| S100A9 | REA859 | 50 | 130-114-515 | FITC | Miltenyi Biotec |
| SSEA-1 (CD15) | REA321 | 50 | 130-117-689 | PE | Miltenyi Biotec |
| TIM3 | REAL818 | 50 | 130-125-682 | PE | Miltenyi Biotec |
| Vimentin | REA409 | 50 | 130-123-774 | PE (APC, FITC) | Miltenyi Biotec |
| VISTA | D1L2G | 200 | 18946 | PE | Cell Signaling Technology |
| β-Actin | REA1148 | 50 | 130-120-276 | FITC | Miltenyi Biotec |
| β-Catenin | REA480 | 50 | 130-123-546 | FITC (APC, PE) | Miltenyi Biotec |