. 2024 Mar 19;12:1351827. doi: 10.3389/fchem.2024.1351827

TABLE 5.

Analgesic activities of crude and various fractions of H. aitchisonii following the hot plate model.

Sample	Dose mg/kg b.wt	Reaction time on the hot plate in seconds
Sample	Dose mg/kg b.wt	15	30	45	60
Negative control	-	3.91 ± 0.52	4.94 ± 0.22	3.32 ± 0.52	2.72 ± 0.40
Ha.Cr	10	6.45 ± 0.87^ns	5.52 ± 0.62^ns	4.67 ± 0.47^ns	3.30 ± 0.74^ns
	25	7.52 ± 0.50*	6.41 ± 0.87^ns	5.50 ± 0.62^ns	4.69 ± 0.49^ns
	50	8.32 ± 0.44***	8.63 ± 0.39**	7.24 ± 0.58*	6.15 ± 0.73^ns
Ha.Hex	10	3.48 ± 0.57^ns	3.34 ± 0.92^ns	2.65 ± 0.32^ns	2.24 ± 0.55^ns
	25	4.15 ± 0.62^ns	3.15 ± 0.74^ns	3.48 ± 0.57^ns	3.14 ± 0.92^ns
	50	6.45 ± 0.64^ns	5.86 ± 0.36^ns	5.62 ± 0.78^ns	4.28 ± 0.45^ns
Ha.Chf	10	8.53 ± 0.62***	8.10 ± 0.20*	7.90 ± 0.30*	6.52 ± 0.52^ns
	25	10.90 ± 0.10***	9.50 ± 0.52***	9.10 ± 0.32***	8.60 ± 0.28**
	50	12.52 ± 0.64***	11.80 ± 0.22***	10.60 ± 0.20***	10.10 ± 0.10***
Ha.EtAc	10	8.20 ± 0.26***	7.88 ± 0.44*	7.10 ± 0.22*	5.96 ± 0.76^ns
	25	9.86 ± 0.50***	8.80 ± 0.44**	8.20 ± 0.46**	8.22 ± 0.28*
	50	11.98 ± 0.32***	11.20 ± 0.22***	10.10 ± 0.52**	9.62 ± 0.52**
Ha.Bt	10	3.66 ± 0.64^ns	4.50 ± 0.92^ns	3.50 ± 0.52^ns	3.73 ± 0.71^ns
	25	5.80 ± 0.32^ns	4.78 ± 0.60^ns	4.50 ± 0.60^ns	4.52 ± 0.82^ns
	50	6.96 ± 0.90**	5.60 ± 0.20^ns	5.90 ± 0.60^ns	5.26 ± 0.53^ns
Ha.Aq	10	4.66 ± 0.81^ns	4.548 ± 0.43^ns	4.50 ± 0.30^ns	3.55 ± 0.96^ns
	25	5.55 ± 0.81^ns	5.69 ± 0.80^ns	4.81 ± 0.44^ns	4.13 ± 0.27^ns
	50	6.92 ± 0.82**	5.80 ± 0.60^ns	5.59 ± 0.33^ns	5.68 ± 0.34^ns
Morphine	5	12.22 ± 0.20***	12.10 ± 0.32***	11.44 ± 0.42***	11.16 ± 0.10***

The data are shown as mean ± SEM (n = 5) for simplicity. The asterisks represent statistically significant differences from the baseline condition: Student’s t-test was used to analyze the data. ^∗ p < 0.05; ^∗∗ p < 0.01, ∗∗∗p < 0.001. ns, not statistically significant; Ha, H. aitchisonii; Cr, crude; Hex, hexane; Chf, chloroform; EtAc, ethyl acetate; Bt, butanol; Aq, aqueous fraction.