REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-PGP9.5 primary antibody	Abcam, Inc., Cambridge, UK	Cat # ab108986
Biotinylated goat antirabbit immunoglobulin G	Vector, Burlingame, CA, USA	Cat # BA-1000
DyLight-488 anti-rabbit IgG	Vector, Burlingame, CA, USA	Cat # DI-1088
Biological samples
Healthy human adult sole skin tissue	This paper	National Taiwan University Hospital (No. 201610037DINC)
Chemicals, peptides, and recombinant proteins
Potassuim Permanganate	Kanto chemical co., Inc.,Japan	Cat # 32373-00
Oxalic acid dihydrate	Kanto chemical co., Inc.,Japan	Cat # 31045-01
Hydrogen Peroxide	Kanto chemical co., Inc.,Japan	Cat # 18084-00
Triton X-100	Sigma-Aldrich Co, USA	Cat # SLBN8333V
Trizma(R) base	Sigma-Aldrich Co, USA	Cat # SLBV9322
Methanol	Sigma-Aldrich Co, USA	Cat # SZBG0150V
Critical commercial assays
Avidin-biotin complex kit	Vector, Burlingame, CA, USA	Cat # PK-6100
Chromogen SG	Vector, Burlingame, CA, USA	Cat # SK-4700
Experimental models: Organisms/strains
Mouse: C57BL/6J	The Jackson Laboratory	Stock #000664
Software and algorithms
ImageJ	National Institutes of Health, USA	https://imagej.net/ij/
MATLAB	MathWorks, USA	https://www.mathworks.com/
ThorImage®LS	Thorlabs, Inc.	https://www.thorlabs.com/
Leica ICC50D	Leica Microsystems Inc.	https://www.leica-microsystems.com/
SPSS software	IBM Corporation, Armonk, NY, USA	https://www.ibm.com/products/spss-statistics
Other
iSpacer	SunJin Lab Co.	Cat # ISO11