TABLE 3.
Pulmonary recruitment of T cells in IFN-γ−/− mice infected with the WSN virus
| Phenotype | No. of cells/lung (105)a
|
|
|---|---|---|
| IFN-γ−/− mice | Wild-type mice | |
| Total CD3+ | 21.5 ± 6.4 | 18.6 ± 4.9 |
| CD3+ CD4+ CD8− | 6.2 ± 2.4 | 7.0 ± 1.9 |
| CD3+ CD8+ CD4− | 8.8 ± 2.9 | 7.3 ± 2.0 |
| CD3+ CD4− CD8− | 6.5 ± 1.8 | 4.3 ± 0.9 |
a
Cells were harvested from the lungs of immunized mice at day 3 after the heterologous challenge, depleted of plastic-adherent cells, and stained for CD3, CD4, and CD8 antigens. The cell number for each subset was estimated in groups of three animals while taking into account the total number of cells separated per lung and the percentage of the particular subpopulation. Results are expressed as means ± SEM.