Table 3.
Primary murine monoclonal antibodies used in immunohistochemical stains.
| Antibody* | Clone | Company | Dilution | Cell type | Staining pattern |
|---|---|---|---|---|---|
| Immunoprofiling | |||||
| CD3 | 2GV6 | Ventana Medical Systems | pre-diluted | T-cells | membranous |
| CD4 | SP35 | Ventana Medical Systems | pre-diluted | T-helper cells | membranous |
| CD8 | SP57 | Ventana Medical Systems | pre-diluted | cytotoxic T-cells | membranous |
| CD25 | 4C9 | Ventana Medical Systems | pre-diluted | regulatory T-cells | membranous |
| FOXP3 | EP340 | MEDAC/BSB | 1:100 | regulatory T-cells | nuclear |
| CD20 | L26 | Ventana Medical Systems | pre-diluted | B-cells | membranous |
| CD68 | KP-1 | Ventana Medical Systems | pre-diluted | macrophages | membranous |
| HSP60 | |||||
| GroEL (bacterial HSP60) | A57-B9 | Alexis Corporation | 1:500 | Chlamydia trachomatis HSP60 (A57-B9) | membranous |
| HSP60 | 611959 | BD Transduction Laboratories ™ | 1:600 | human HSP60 | membranous |
*All antibodies are CE-IVD certified.