Figure 5.

IRF8 ^G388S promotes increased osteoclast activity and tooth root resorption in the dentoalveolar region. Histological analyses of maxillae by immunohistochemical (IHC) staining for CTSK demonstrate increased osteoclast activity (arrows indicate osteoclasts) and root resorption in oral ligature–inserted Irf8 KI Het and Homo mice compared to wild-type (WT) mice. Bar graph shows quantified CTSK⁺ osteoclast numbers. n = 5 mice per genotype (2 males and 3 females in each group; circle denotes male mice, and triangle denotes female mice). The data are presented as the mean ± SD, and Het and Homo mice were compared against WT mice. One-way analysis of variance and post hoc Tukey’s test was used for comparisons among groups.