Case 13S. Automatic gain function may contribute to false positives. The visible-light image (A), NIR fluorescence superimposed and color-mapped onto the visible-light view (B), and NIR image (C) reveal the main specimen displaying strong NIR signal with a gain percentage at 15%. The visible-light image (D), NIR fluorescence superimposed and color-mapped onto the visible-light view (E), and NIR image (F) obtained after resection of the main tumor, showing a gain percentage increase to 71%. Notice the presence of a significant increase in background NIR signal in the skin edges partially obscured by Raney clips, bone, and so forth. Biopsy did not confirm the presence of meningioma.