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实时荧光定量PCR引物及退火温度
| 基因 | 序列(5’~3’) | 退火温度/℃ |
| E-cadherin | F: TCACTGACACCAACGATAATCC R: TTTCAGTGTGGTGATTACGACGTTA |
60 |
| Piezo1 | F: TGGAGGAGGCTGGCATCATCTG R: GACGTGCAGGTAGTAATGGCTAAGG |
60 |
| Vimentin | F: CCGAAAACACCCTGCAATCTTTC R: CACATCGATTTGGACATGCTGT |
60 |
| GAPDH | F: TGACATCAAGAAGGTGGTGAAGCAG R: GTGTCGCTGTTGAAGTCAGAGGAG |
60 |
