Table 5:
qPCR cycling conditions.
| Steps | Temperature | Time | Cycles |
|---|---|---|---|
| Initial denaturation | 98 °C | 30 s | 1 |
| Denaturation | 98 °C | 10 s | 20 |
| Annealing | 63 °C | 30 s | |
| Elongation | 72 °C | 1 min | |
| Hold | 4 °C | Forever |
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